Effect of Cryoprotectants on Viability of Canine Spermatozoa

摘要

The semen was cryopreserved and the post-thaw evaluation was performed after 24 h of freezing. The sperm viability, membrane integrity and DNA integrity test were performed in pooled fresh semen and post-thaw semen besides regular, conventional semenevaluation. Five per cent ethylene glycol is a significantly (P<0.01) better cryoprotectant in terms of higher livability, lesser abnormality, higher HOSST and acrosomal integrity.