Electrochemical Response of β-Galactosidase- and Glucose Oxidase-containing Microcapsule-immobilized Electrode

摘要

A polyamide microcapsule containing bi-enzyme, β-galacto-sidase (β-Gal) and glucose oxidase (GOD), was prepared by interfacial polymerization. The average diameter of the microcapsule was evaluated to be 5 urn. The bi-enzyme-containing microcapsule was immobilized with tetrathiafulvalene on a carbon electrode surface and was characterized by cyclic voltammetry. The oxidation current increased with the increase of the lactose concentration. It was concluded that β-Gal and GOD retained their enzymatic activity in the microcapsule and reacted with lactose, and tetrathiafulvalene penetrated the polyamide membrane.