Synthesis of Staudihger-type Molecular Probe for Catch-and-release Purification of the Binding Protein for Potassium Isolespedezate,a Leaf-closing Substance of Leguminous Plant

摘要

We synthesized azide-containing photoaffinity probe 1 based on the structure of potassium isolespedezate.This probe can be used for catch-and-release-mechanism purification of binding protein for 1:photo-crosslinking with 1 gave azide-la-beled receptor which can be captured by phosphane-linked gel matrix by the Staudinger ligation.After washing the gel,the caught binding protein can be released by the reductive cleavage of disulfide bond in 1.This process can be used as a convenient method for the purification of binding protein for bioactive natural product.