Poultry meat speciation by sequence analysis of mitochondrial 12s rRNA gene.

摘要

Sequence analysis of mitochondrial (mt) 12S ribosomal RNA gene was applied for species identification of chicken (Gallus gallus), duck (Anas platyrhynchos), domestic quail (Coturnix japonica), turkey (Meleagris gallopavo) and guinea fowl (Numida meleagris) meat. The method involves DNA extraction, polymerase chain reaction (PCR) amplification of mt 12S rRNA gene, sequencing of amplicons followed by sequence alignment and analysis. Successful PCR amplification was achieved in both fresh and processed meat products. Sequence analysis could decisively distinguish closely related species such as chicken-quail and turkey-guinea fowl. The sequence analysis of mt 12S rRNA gene can be successfully exploited for poultry meat species identification.