High frequency plant regeneration from an amphiploid hybrid seed of Glycine max x G. tomentella through tissue culture and hybridity testing of the regenerants.

摘要

Fifty-five plants from the amphiploid hybrid (2n=118) of Glycine max (2n=40) and G. tomentella (2n=78) were regenerated from a single seed in tissue culture. The embryonic axis converted into a solid green mass and gave rise to shoot buds on benzyladenine-supplemented medium. Shoots were eventually obtained on the same medium. Rooting occurred in 100% of the plants on Whites medium containing coumarin (9 mg/litre). A total of 50 plants survived the hardening treatment. All of the tissue culture-raised plants were morphologically similar and flowered under greenhouse conditions. The hybridity of the regenerated plants was confirmed using morphological and simple sequence repeat (SSR) markers. Attempts are being made to cross these plants with soyabean to generate backcross-derived lines for further genetic studies..