Intraspecific and gender variation in hmcp4 gene of cysteine proteinase in Haemonchus contortus.

摘要

Different types of cysteine proteinases, viz. hmcp 1, hmcp4 and hmcp6 reported in U.K. strain of Haemonchus contortus as well as in USA gcp-7, are incriminated in the expression of gut enzymes. In the Indian isolate of H. contortus, information on the presence of these genes is lacking. During the present study intraspecific and gender variations of hmcp4 gene was conducted. The gene was cloned and characterized in the 2 commonly occurring morphotypes of females having buttonform and linguiform vulva as well as male worms. RNA was isolated from 2 morphotypes of adult female and male H. contortus, separately. The cDNA was synthesized by reverse transcription and the hmcp4 gene was PCR amplified using Taq DNA polymerase. The amplified PCR product was purified and cloned. Sequence homologies between buttonform female and linguiform female H. contortus with respect to hmcp4 gene in U.K. strain were 100% and 98.8%, respectively whereas with male H. contortus the homology was 98.3%. Intraspecific as well as gender specific variation were also recorded with respect to hmcp4 gene of cystein proteinase in H. contortus for the first time. Amino acid sequence homology between male and female H. contortus of Indian isolate and published U.K. strain were also recorded.