Factors influencing the establishment of haploid embryogenic callus from anthers in Jatropha curcas (L.).

摘要

An experiment was conducted to study the various factors for commencement of embryonic callus line from anthers in Jatropha curcas. Flower buds of were procured from mature J. curcas plants (6-years old) growing in Rajasthan College of Agriculture, Udaipur. The phenological correlation between size (length) of flower buds and required developmental stage of pollen was determined. At 6% sucrose concentration, 70.3% of anthers showed callus covering the entire anther surface. Severe browning and death of anther cultures was recorded on media combined with maltose and sucrose at 4% level. Cold pretreatment of 3 days of flower buds were promising for induction of haploid embryonic callus on N₆ media supplemented with 1.0 mg/litre 2,4-D + 0.5 mg/litre kinetin + 6% sucrose.