Genetic polymorphism in Jakhrana goats and their comparison with other Indian goat breeds through RAPD

摘要

RAPD markers were used to estimate the genetic variability of Jakhrana breed with other extant goat breeds, and to evaluate genetic relationship between them. Adult females, 15 from each breed (Jakhrana, Barbari, Sirohi, Black Bengaland Jamunapari), were randomly selected. Polymorphic random primers OPM-12, OPM-18, OPM-14, OPA-01, OPM-05, OPP-08, OPP-02 were used for analysis. The estimates were based on band sharing proportion and based on the band frequency. When pooled over the primers, based on band sharing, Black Bengal (0.890) showed minimum while Jakhrana showed maximum (0.994) within-breed genetic similarity. Similar trend was observed based on band frequency where Black Bengal (0.800) showed minimum and Jakhrana showed maximum (0.890) within breed genetic similarity. For between-breed similarities, based on band sharing, genetic similarity was observed minimum between Jamunapari and Jakhrana (0.846) while maximum was observed between Sirohi and Jakhrana (0.994), based on band frequency, the minimum was observed between Jamunapari and Sirohi (0.985), while Black Bengal and Barbari showed maximum genetic similarity of 0.999. These results can be utilized to upgrade and improve the breeds which are genetically close through a common breed improvement program.