CIRCULATING OVARIAN STEROID PROFILES IN SURTI BUFFALOES SUPEROVULATED WITH FSH AND DIFFERENT DOSES OF PMSG

摘要

Eighteen Surti buffaloes of 2 to 6 lactations were superovulated during breeding season (August to February) using FSH and 2 doses of PMSG. Animals were divided into three groups. Group I (n=6) and Group II (n=6) donors were superovulated with singlei/m dose of 2500 and 3000 IU PMSG, respectively, on day 10 of the estrous cycle, whereas Group III (n=6) donors were treated intramuscularly with 400 mg FSH per donor in 8 equal doses 12 hours apart for four days. Jugular samples were collected from alldonors at five different stages of MOET, i.e, Day 0 (Heat), Day 10 (PMSG treatment), Day 12 (2 PGF_(2alpha) inj.), Day 14 (Superovulatory estrus) and Day 20 (Flushing) for serum separation. No significant variation was found in serum concentrations of progesterone and estradiol-17ss among three groups of donors during various stages of MOET. However, the serum concentrations of progesterone and estradiol-17ss at various stages of MOET within the group varied significantly (p<0.05). The number of CLs hadsignificant positive correlations with the serum progesterone (r = 0.125) and estradiol-17ss (r = 0.482) profile on pooled basis. The mean serum concentrations of progesterone were found to be highest on the day of embryo collection (1.83 +- 0.63; 3.01+-0.23 and 3.35 +-0.68 ng/ ml) in 3 groups (Group I, II and III, respectively) of donors, whereas the mean serum concentrations of estradiol-17ss were found to be the highest on the day of superovulatory estrus (42.67 +- 5.44; 46.17 +-6.27 and 50.17 +- 7.12 pg/ml, respectively).