Standardization of regeneration protocol for small flower chrysanthemum (Chrysanthemum morifolium) cultivars.

摘要

In the present investigation five different cultivars of Chrysanthemum morifolium were screened using the ray floret explants in order to determine the capability for plant regeneration on four media protocols and consequently to find out the best genotype source linked with the optimum medium conditions for the high potentiality of shoot formation. The results indicated that all in vitro culture traits were significantly influenced by the differences in cultivars, medium protocols and their interaction. The percentage of explants which developed in to calli ranged from 34.1% to 47.2% among five different cultivars across the four protocols with an average of 40.80%. The highest percentage of embryogenic callus, shoot formation and mean value of shoot length was produced by cultivar Purnima when calli were differentiated on protocol A. The protocol A showed the greatest potential for shoot length across the cultivars and it was significantly superior to all other protocols except the protocol B. Results indicated that the protocol A, followed by B appear to be the best protocols for plant regeneration and it can be used by the breeders in chimera management to establish the new cultivar in a better way.