pH-Dependent Dissociation of Carbohydrate Ligand Polycations from DNA Ternary Complexes

摘要

Here,we have demonstrated that carbohydrate ligand poly-cations are pH-dependently dissociated from DNA polyion complexes.In response to pH,DNA ternary complexes have dissociated the ligand polycations,resulting in DNA binary complexes.Namely,the ternary complexes of DNA,a poly(L-histidine) modified with aminoethyl groups,PLH-NH_2,and a poly(L-ly-sine) conjugated with lactose molecules,PLL-Lac,at pH 7.5 have dissociated the PLL-Lac polycations by the protonation of imidazole groups of PLH-NH_2 at pH 6.0.The pH-dependent dissociation of the ligand polycations from DNA polyion complexes at endosomal pH is expected to offer unique design for DNA delivery systems.