Role of Cytokinins and Explant Type in Shoot Multiplication of Buckwheat (Fagopymm esculentum Moench) in- Vitro

摘要

The main objective of micropropagation after in vitro culture establishment is to work out an efficient and reliable method for shoot multiplication. The effect of isoprenoid (2-iP) and aromatic cytokinins (BA, BAR, KIN, TOP) were tested in experiments aimed on shoot multiplication. Beside the evaluation of the effects of different types and concentrations (1 to 20 mu M) of cytokinins, the influence of explant type (shoot tip, nodal segment) on efficient multiplication was also assessed. Both type and concentration of applied cytokinins and the type of explants had a strong effect on the shoot multiplication and on growth type of in vitro shoots. A very tight relationship between the cytokinins and explant types was observed. If meta-topolin was applied between 5 and 15 uM and shoot tips were used as initial explants, high multiplication (3.05-3.49 shoots and 13.26-14.42 nodes per explant) could be achieved and the mean length of shoots was satisfactory (> 40 mm). Results obtained may contribute tothe development of an efficient micropropagation protocol for common buckwheat.