Ouabain-Sensitive and Ouabain-Resistant Isoforms of Na~+,K~+-ATPase in Cerebellar Granule Cells Control MAP Kinase Activity

摘要

Three-hour incubation of rat cerebellar granule cells with 0.1 μM ouabain increases intracellularlevels of Ca~(2+)ions and reactive oxygen species (ROS) resulting in pronounced activation of Mitogen-ActivatedProtein Kinase (MAPK). Higher concentrations of ouabain induce further increases in MAPK activity. The acti-vating effect of ouabain is attenuated by the NMDA-receptor antagonists MK-801 and D-AP5. The data obtainedsuggest that similar to NMDA receptors ouabain-sensitive and ouabain-resistant isoforms of Na~+,K~+-ATPase areactively involved in intracellular signaling cascades controlling proliferative activity of neuronal cells.