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首页> 外文期刊>The Biochemical Journal >CHRONIC EXOGENOUS HYPERINSULINAEMIA DOES NOT MODIFY THE ACUTE INHIBITORY EFFECT OF INSULIN ON THE SECRETION OF VERY-LOW-DENSITY LIPOPROTEIN TRIACYLGLYCEROL AND APOLIPOPROTEIN B IN PRIMARY CULTURES OF RAT HEPATOCYTES
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CHRONIC EXOGENOUS HYPERINSULINAEMIA DOES NOT MODIFY THE ACUTE INHIBITORY EFFECT OF INSULIN ON THE SECRETION OF VERY-LOW-DENSITY LIPOPROTEIN TRIACYLGLYCEROL AND APOLIPOPROTEIN B IN PRIMARY CULTURES OF RAT HEPATOCYTES

机译:慢性外源性高胰岛素血症不能改变胰岛素对大鼠肝细胞原代培养中非常低密度脂蛋白三环甘油和载脂蛋白B分泌的急性抑制作用

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摘要

Male Wistar rats were fitted with subcutaneous osmotic minipumps that delivered insulin at a constant rate of 0.20 i.u./h for 7 days. This treatment raised the plasma insulin concentration from 31 +/- 4 to 201 +/- 64 mu-i.u./ml. Hepatocytes prepared from the hyperinsulinaemic animals secreted very-low-density lipoprotein (VLDL) triacylglycerol (TAG) at a higher rate (172 +/- 21 mu g per 24 h per mg cell protein) than did those from sham-operated controls (109 +/- 12 mu g per 24 h per mg) (P < 0.05). However, chronic exogenous hyperinsulinaemia had no stimulatory effect on the secretion of VLDL apolipoprotein B (apoB) in derived hepatocytes compared with those from the sham-operated controls (2.32 +/- 0.38 compared with 3.09 +/- 0.40 mu g per 24 h per mg). Hepatocytes from the hyperinsulinaemic rats thus secreted larger VLDL particles as evidenced by the increased TAG:apoB ratio (78.4 +/- 13.1 compared with 38.4 +/- 7.6; P < 0.05). In hepatocytes from the hyperinsulinaemic rats a larger proportion of the newly synthesized TAG was secreted as VLDL. Hepatocytes from the hyperinsulinaemic and the sham-operated control animals were equally sensitive to the inhibitory effect of insulin added in vitro on the secretion of VLDL TAG. Insulin added in vitro to the culture medium of hepatocytes from hyperinsulinaemic animals significantly decreased the TAG:apoB ratio of the secreted VLDL. This change did not occur in hepatocytes from sham-operated rats. These results suggest that, in vivo, chronic hyperinsulinaemia is not in itself sufficient to desensitize the liver to the acute inhibitory effect of insulin on the secretion of VLDL. [References: 49]
机译:雄性Wistar大鼠装有皮下渗透微型泵,该泵以0.20 i.u./h的恒定速率输送胰岛素达7天。该处理将血浆胰岛素浓度从31 +/- 4提高至201 +/- 64μi.u./ ml。从高胰岛素血症动物制备的肝细胞分泌的超低密度脂蛋白(VLDL)三酰甘油(TAG)的分泌速率(每24小时每毫克细胞蛋白172 +/- 21微克)要比假手术对照组的更高(109)每24小时每毫克+/- 12微克(P <0.05)。然而,与假手术对照组相比,慢性外源性高胰岛素血症对衍生肝细胞中的VLDL载脂蛋白B(apoB)的分泌没有刺激作用(2.32 +/- 0.38,而每24 h为3.09 +/- 0.40μg毫克)。因此,来自高胰岛素血症大鼠的肝细胞分泌出更大的VLDL颗粒,这由TAG:apoB比率的增加所证实(78.4 +/- 13.1,而38.4 +/- 7.6; P <0.05)。在高胰岛素血症大鼠的肝细胞中,新合成的TAG的较大部分被分泌为VLDL。高胰岛素血症动物和假手术对照组动物的肝细胞对体外添加的胰岛素对VLDL TAG分泌的抑制作用同样敏感。体外向高胰岛素血症动物的肝细胞培养基中添加胰岛素可显着降低分泌的VLDL的TAG:apoB比。在假手术大鼠的肝细胞中未发生这种变化。这些结果表明,在体内,慢性高胰岛素血症本身不足以使肝脏对胰岛素对VLDL分泌的急性抑制作用不敏感。 [参考:49]

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