Determination of 4-nonylphenol and 4-octylphenol in human blood samples by high-performance liquid chromatography with multi-electrode electrochemical coulometric-array detection

摘要

Alkyiphenols can affect human health because they disrupt the endocrine system. In this study, an analytical method for determining trace amounts of 4-nonytphenol (NP) and 4-octyiphenol (OP) in human blood samples was developed. Reversed-phase HPLC with multi-electrode electrochemical coulometric-array detection was used for the determination of NP and OP in plasma and serum samples prepared with a solid-phase extraction method. The separation was achieved using an isocratic mobile phase of 0.7% phosphoric acid—acetonitrile with a C,s reversed phase column. The detection limits of NP and OP were 1.0 and 0.5 ng mV’, respectively. The recoveries of NP and OP added to human plasma samples were above 70.0% with a relative standard deviation of less than 15.5%. The method was found to be applicable to the determination of NP and OP in various human blood samples such as serum and plasma.