Fusion expression of bovine lactoferricin in Escherichia coli

摘要

The drug-resistance problem has been growing with use of current antibiotics in the feed and medical industries. LfcinB, a 25 amino acid antibacterial peptide derived from bovine lactoferrin, is a potential alternative to antibiotics. According to the bias of codon use in Escherichia coli, the gene encoding LfcinB has been chemically synthesized and ligated into the expression vector pGEX-4T-2. The antibacterial peptide was fused with GST with a protease cleavage site in between them. Two constructs with different cleavage sites were made. One construct, GEX-Th-LCB, contained a thrombin cleavage site provided by the vector.