On Plate Analysis of Surfactants by High Performance Thin-Layer Chromatography:Simultaneous Identification and Separation of Polyoxyethylene p-t-Octyl Phenol,Dodecyltrimethylammonium Bromide and Polyoxyethylene(20) Stearyl Ether

摘要

Thin - layer chromatography of fifteen surfactants (cationic,anionic and non - ionic) has been performed on silica HPTLC plates using chromogenic reagents methylene blue (MB),brb-mocresol green (BCG),and bromophenol blue (BPB) in the presence of aqueous formic acid plus acetone.Mobile phase system comprising acetone + 10~4 M bromophenol blue in 1 M aqueous formic acid is found most favourable for the separation of coexisting,polyoxyethylene p-t-octylphenol (TX-100),poly-oxyethylene(20) stearyl ether (Bj-78),and dodecyltrimethylammonium bromide (DTABr),Effects of sample pH,presence.of impurities in the analyte sample,nature of adsorbent and the composition of mobile phase have been investigated on the separation of TX-100,Bj-78,and DTABr from their mixtures.The limits of detection of poly oxyethylene p-t-octylphenol,polyoxy-ethylene(20) stearyl ether and dodecyl trimethyl ammonium bromide have been determined.