Asymmetric hydrolysis of enol esters with two esterases from Marchantia polymorpha

摘要

Two esterases participating in the asymmetric hydrolysis of a-alkylated enol acetates to a-chiral ketones were isolated from the cultured cells of Marchantia polymorpha. These two esterases had opposite enantioselectivities and both of them reversed the stereoselectivity of protonation into the enol intermediate in the hydrolysis when the chain length and the bulkiness of a-substituents were increased. (C) 2000 Elsevier Science Ltd. All rights reserved. [References: 25]