Determination of isosorbide dinitrate in arterial plasma, synthetic serum and pharmaceutical formulations by linear sweep voltammetry on a gold electrode

摘要

Isosorbide dinitrate (ISDN) is reduced voltammetrically at gold working electrode surface in aqueous sodium sulfite solution and produces a voltammogram with its peak current proprotional to the concentration of ISDN in the range of 1.3-2340 #mu#g ml~(-1). Sodium sulfite is used as supporting electrolyte and oxygen removing agent and, therefore, no nitrogen gas purging for elimination of soluble oxygen in, the sample solution is required. The limit of detection (LOD) of the method is 0.0838 #mu#g ml~(-1) and the relative standard deviation (R.S.D.) of 24 replicate determination of 52 #Mu#g ml~(-1) of ISDN is 3.60%. The method is used for quantitative analysis of ISDN in arterial plasma, synthetic serum and pharmaceutical dosage form. The results are compared with those obtained from GC determination method. The method is sensitive and requires little sample preparation in a wide concentration range.