Reversed-phase ion-pair high performance liquid chromatographic determination of Co(Ⅲ)-,Ni(Ⅱ)-, V(V)- and (Ⅲ)-2-(2-benzothiazolylazo)-5-(3-sulforpropyl)aminophenol Chelates

摘要

The separation and determination of Co(Ⅲ). Ni(Ⅱ). V(v) and Fe(Ⅲ) chelates with 2-(2-benzothiazolylazo-5-(3-sulfopropyl)aminophenol (BTASPAP) by reversed-phase ion-pair HPLC was investigated. In the presence of the oxidant potassium iodate. BTASPAP reacts with Co(Ⅲ). Ni(Ⅱ). V(V) and Fe(Ⅲ) to form stable, negatively charged. water-soluble chelates. The chelates were separated on a C↓(18) siloxane bonded phase and eluted within7 min with acetonitrile-acetate-water (36:1:63 v v) containing 0.2 mol1↑(-1) acetic acid-sodium acetate buffer (pH 3.0) and 1.0 mmol 1↑(-1)tetrabutylammonium bromide. The detection limits of Co(Ⅲ) Ni(Ⅱ). V(V) and Fe(Ⅲ) at 565 nm are 0.3, 0.8, 0.3 and 1.0 ng (signal-to-noise ratio = 2). respectively. The method was applied to the determination of Co. Ni, V and Fe in four samples of standard alloys, # 1997 Elsevier Science B. V.