Molecularly imprinted solid-phase extraction for the selective determination of 17 beta-estradiol in fishery samples with high performance liquid chromatography

摘要

A molecularly imprinted polymer(MIP)has been synthesized by a thermo-polymerization method using methacrylic acid(MAA)as functional monomer,ethylene glycol dimethacrylate(EGDMA) as cross-linker, acetonitrile as porogenic solvent,and 17 beta-estradiol as template. The MIP showed obvious affinity for 17 beta-estradiol in acetonitrile solution,which was confirmed by absorption experiments.After optimization of molecularly imprinted solid-phase extraction(MISPE) conditions,three structurally related estrogenic compounds(17 beta-estradiol,estriol. and diethylstilbestrol)were used to evaluate the selectivity of the MIP cartridges.The MIP cartridges exhibited highly selectivity for E-2,the recoveries were 84.8+/-6.53% for MIPs and 19.1 +/-1.93% for non-imprinted polymer(NIP)cartridges.The detection and quantification limits correspond to 0.023 and 0.076 mg L-1. Furthermore,the MISPE methods were used to selectively extract E-2 from fish and prawn tissue prior to HPLC analysis. This MISPE-HPLC procedure could eliminate all matrix interference simultaneously and had good recoveries (78.3-84.5%).