Separation and simultaneous determination of cobalt, nickel and copper with 2-(4-methyl-2-quinolylazo)-5-diethylaminophenol by reversed-phase high-performance liquid chromatography

摘要

Performance of six quinolylazo compounds was studied as a pre-column derivatizing reagent for the separation of metal ions in the reversed-phase high-performance liquid chromatography. 2-(4-Methyl-2-quinolylazo)-5-diethyl-aminophenol (QADP) was suitedfor the determination of Co. Ni and Cu in the presence of a large amount of Fe. The detection limits under the optimized conditions [stationary phase: L-column ODS: mobile phase: acetone-water (62:38. v/v) containing 0.01 M NH_4SCN and 0.01 M 2-morpholinoethanesulfonic acid-NaOH (pH 6): injection volume: 10 μl] were 0.24 ng for Co. 0.22 for Ni and 0.53 for Cu. This method was successfully applied to the determination of these metal ions in a standard steel sample.