首页> 外文期刊>Talanta: The International Journal of Pure and Applied Analytical Chemistry >Miniaturized protein microsequencer with PTH amino acid identification by capillary electrophoresis Ⅱ. A syringe-pump-based system for covalent sequencing
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Miniaturized protein microsequencer with PTH amino acid identification by capillary electrophoresis Ⅱ. A syringe-pump-based system for covalent sequencing

机译:毛细管电泳鉴定PTH氨基酸的微型蛋白质微型测序仪Ⅱ。基于注射泵的共价测序系统

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摘要

A miniaturized protein and peptide microsequencer consisting of a fused silica capillary' reaction chamber is described. Extremely small volumes of reagents, 2 μl or less, were delivered directly to the reaction chamber through individual fused silica capillary lines using Iow pressure syringe pumps. Other than an argon gas controller, no valves were used in the delivery system. The short flow path and very low dead volume were achieved by directly connecting the narrow-bore capillaries to the reaction chamber. This configuration minimized side reactions. The elimination of as well as the use of capillaries for the reaction chamber and delivery lines, greatly simplified the construction ,sequencer. The performance of the sequencer was evaluated by sequencing 8-33 picomoles of myoglobin and chain B that were covalently attached to Sequelon-DITC and Sequelon-AA membranes. # 1997 Elsevier B.V.
机译:描述了由融合的二氧化硅毛细管的反应室组成的微型化的蛋白质和肽微测序仪。使用低压注射泵通过单独的熔融石英毛细管将极少量的试剂(2μl或更少)直接输送到反应室。除氩气控制器外,输送系统中未使用任何阀门。通过将窄口毛细管直接连接到反应室,可以实现短的流路和极低的死体积。这种配置使副反应最小化。消除了反应室和输送管线中的毛细管,并减少了毛细管的使用,大大简化了结构,定序器。通过对与Sequelon-DITC和Sequelon-AA膜共价连接的8-33皮摩尔的肌红蛋白和链B进行测序,可以评估测序仪的性能。 #1997 Elsevier B.V.

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