Quantification of components in overlapping peaks from capillary electrophoresis by using continues wavelet transform method

摘要

The application of continues wavelet transform (CWT) for resolving overlapping peaks from capillary electrophoresis (CE) is described. Overlapping peaks can be resolved easily by transforming experimental signals into their wavelet coefficients. The proposed method was applied for the determination of benzoic acid and salicylic acid in overlapping peaks from CE. The composition of the two acids in Zuguangsan, a traditional Chinese patent medicine, was determined. The quantification results are satisfactory. CWT has been shown to be a practicable approach for resolving overlapping peaks and for quantitative determining coeluted compounds in overlapping peaks from CE. The quantification results obtained-from CWT were compared with those obtained from numerical differentiation method. CWT has been shown prior to numerical differentiation method for processing experimental signals which contain noise. (c) 2008 Elsevier B.V. All rights reserved.