DETECTION OF BAT CORONA VIRUS AND SPECIFIC ANTIBODIES IN CHESTNUT BAT (SCOTOPHILUS KUHLII) POPULATION IN CENTRAL TAIWAN

摘要

Bats can serve as natural reservoirs for severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome coronavirus (MERS-CoV). Investigating the prevalence of bat CoV is critical for assessing the risks of the outbreaks of emerging CoV. Chestnut bats (Scotophilus kuhlii) were captured in this study for detecting the partial RNA-dependent RNA polymerase (RdRp) gene in their feces through reverse transcription polymerase chain reaction (RT-PCR) and antibodies to the nucleocapsid (N) protein of bat CoV through western blotting (WB) analysis. Three recombinant N protein fragments (Nl, N2, N3) of the isolated Scotophilus bat CoV/CYCU-Sl/TW/2013 were expressed by Escherichia coli. WB analyses were performed with bat serum samples and the sera of a patient who recovered from a SARS-CoV infection. Fragment N2 contained a highly conserved motif among CoVs whereas Nl and N3 protein fragments were specific to the S. kuhlii bat CoV. A total of 32 fecal and 19 serum samples were collected in Changhua County and Yunlin County during 2013 and 2014. About 17 fecal samples tested positive for the RdRp gene with an overall prevalence of 53%. Sequences comparison showed that the Scotophilus bat CoV isolates in Taiwan belonged to the genus Alphacoronavirus and were closest to Scotophilus bat CoV/Hainan/China/2005 and Dili-manl552Gl/Philippines/2008, followed by porcine epidemic diarrhea coronavirus. Only one bat serum sample reacted positively to all 3 N protein fragments. Cross-reactivity was observed between N2 protein fragment and the sera of a patient recovered from a SARS-CoV infection. The results indicated that Scotophilus bat CoV was circulating endemically in chestnut bat population in Taiwan.