PROLIFERATIVE EFFECT OF PLATELET-RICH FIBRIN ON CANINE BONE MARROW-DERIVEDSTROMAL CELLS

摘要

Platelet-rich fibrin (PRF) has been widely used to accelerate wound and bone healing in human patients. The aim of the study was to establish a canine bone marrow-derived stromal cell (BMSC) culture system and to investigate the effect of PRF on thesecells. PRF was prepared using density gradient centrifugation and BMSCs were obtained from the fracture sites of 23 dogs during surgical procedures and individually analyzed. Canine BMSCs were either cultured in osteogenic medium or cultured with/without the presence of PRF. Canine BMSCs expressed similar surface antibodies to mesenchymal stem cells and were able to undergo osteogenic differentiation in osteogenic medium. PRF stimulated proliferation of BMSCs, but PRF alone had no effect on osteogenicdifferentiation. This study provides useful information about the proliferation effect of PRF, and the canine culture system in the presence of osteogenic medium allows the differentiation of BMSCs in vitro. These results may benefit future application in the clinic.