Advantages and Potential of Lipid-Membrane-Incorporating Fullerenes Prepared by the Fullerene-Exchange Method

摘要

Lipid-membrane-incorporating C_(60) and C_(70) (LMIC_(60) and LMIC_(70)) were prepared by the fullerene-exchange reaction from the gamma-cyclodextrin cavity to vesicles (we call this method the "exchange method"). An advantage of this method is that the ratios of [C_(60)]/[lipids] and [C_(70)]/[lipids] can be arbitrarily controlled by adjusting the ratios of the fullerenes and liposome. The maximum ratio (30 mol percent) obtained was approximately 14 and 100 times higher than those achieved for LMIC_(60) and LMIC_(70), respectively, that were prepared by the classical method, which we call the "premixing method" (dissolving lipids and C_(60) or C_(70) in chloroform, followed by concentration and extraction with water). Furthermore, the stabilities and photodynamic activities of the LMIC_(60) and LMIC_(70) solutions prepared by the exchange method were shown to be much higher than those prepared by the premixing method. That is, the exchange method was found to be superior to the premixing method as a preparative method of LMIC_(60) and LMIC_(70) for applications in photomedical and photomaterials chemistry.