Determination of enantiomeric bioactives using new analytical SFC instruments

摘要

Manuscripts dealing with the new analytical SFC devices and comparing analytical HPLC with analytical SFC for enantiomeric separations are reviewed. Supercritical fluid chromatography (SFC) has long been a common tool for preparative separation of enantiomers in science and industry, since it offers some major advantages over high performance liquid chromatography (HPLC). For example SFC allows for higher flow rates, shorter analysis and total run times and usage of longer columns resulting in higher resolution. Recently new SFC instruments became available for analytical application with major improvements in terms of back pressure regulation. This led to enormous progresses in robustness and analytical performance.