Synthetic peptide purification Are isocratic elution and volume overload options to improve process economics?

摘要

In theis paper we use a crude sarnple of bradykinin to demonstrate that synthetic peptides can be purified using isocratic eiution. As the peptide is readily soluble, the purification was carried out using mass overload and the purity and recovery of the peptide determined using high performance analytical HPLC. A second purification was carried out using volume overload with a step gradient to assess the viability of using isocratic eluton for the purification of dilute solutions of crude peptides without the need for a pre-concentration step. The purity and recovery of the two methods is compared.