High speed and high resolution liquid chromatography in practice

摘要

The availability of HPLC columns with very small particles (typically <2 μm) in combination with HPLC pumps with a high pumping capability (>400 bar) opens new possibilities to increase the speed of analysis as well as to improve the chromatographic separation. In this article practical aspects of high speed and high resolution HPLC are discussed and examples are presented that were derived from daily laboratory practice. It is shown that the speed of analysis can be increased by a factor of 5 -10. But also extremely high resolution can be obtained as is shown by a separation on a 2.25 m HPLC column with an apparent plate number as high as 1,000,000.