Immunological Biochips for Parallel Detection of Surface Antigens and Morphological Analysis of Cells

摘要

A biochip for detecting 26 cluster differentiation (CD), HLA-DR and IgM antigens on lymphocytesurface is described. The biochip, which represents a microarray of antibodies (IgG) against a panel of selectedantigens immobilized on transparent plastic surfaces in 1.5-mm spots, was used for the study of normal andneoplastic lymphocytes and can also be used for determining percent of cells expressing definite surface anti-gens in lymphocyte suspensions. The results are consistent with data obtained by flow cytometry. The novelbiochip technology entails a combination of conventional staining of cells immobilized on biochips and mor-phological analysis.