Molecularly imprinted solid-phase extraction of urinary trans-trans muconic acid and analysis by gas chromatography-mass spectrometry [Extra??o de ácido trans-trans muc?nico urinário com polímeros de impress?o molecular e análise por cromatografia gasosa - Espectrometria de massas]

摘要

This paper describes selective molecularly imprinted solid-phase extraction of ttMA from urine samples followed by derivatization and analysis by gas chromatography/mass spectrometry (GC/MS). The analytical calibration curve ranged from 0.3 to 7.0 mg L-1 (r = 0.999) and the limit of quantitation (LOQ) was 0.3 mg L-1. The method was applied for the determination of ttMA in urine samples from smokers and concentrations detected ranged from < LOQ to 1.64 mg L-1. Thus, the proposed method proved adequate for the determination of urinary ttMA in the biomonitoring of occupational exposure to low levels of benzene.