Qualitative and quantitative mycotoxin analyses in residual water of rice production chain by TLC and HPTLC [Análises quali e quantitativas de micotoxinas em águas da cadeia produtiva do arroz por CCD e CCDAE]

摘要

This study validated a simple and applied method for determining mycotoxins aflatoxin B_1, aflatoxin B_2, ochratoxin A, zearalenone and deoxynivalenol, in water from the rice production chain. Five solvent combinations for extraction were tested, with quantification performed by TLC/HPTLC and confirmation by LC-MS/MS. Mycotoxins in water from field and rice industries were evaluated. Mycotoxin recovery levels were around 90%. Two samples from rice parboiling waste were contaminated (deoxynivalenol/aflatoxin B_1, 110/9 ng mL~(-1); and deoxynivalenol, 100 ng mL~ (-1)). Zearalenone, deoxynivalenol and ochratoxin A (36, 30 and 28%) were carried to soaking water during parboiling.