ENANTIOSELECTIVE BIOTRANSFORMATION OF PROPRANOLOL TO THE ACTIVE METABOLITE 4-HYDROXYPROPRANOLOL BY ENDOPHYTIC FUNGI

摘要

The enantioselective biotransformation of propranolol (Prop) by the endophytic fungi Phomopsis sp.. Glomerella cingulata, Penicillium crustosum. Chaetomium globosurn and Aspergillus fumigatus was investigated by studying the kinetics of the aromatic hydroxylation reaction with the formation of 4-hydroxypropranolol (4-OH-Prop). Both Prop enantiomers were consumed by the fungi in the biotransformation process, but the 4-hydroxylation reaction yielded preferentially (-)-(S)-4-OH-Prop. The quantity of metabolites biosynthesized varied slightly among the evaluated endophytic fungi. These results show that all investigated endophytic fungi could be used as biosynthetic tools in biotransformation processes to obtain the cnantiomers of 4-OH-Prop.