DETERMINATION OF SILDENAFIL CITRATE AND TADALAFIL BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE-ARRAY DETECTION (UPLC-DAD)

摘要

DETERMINATION OF SILDENAFIL CITRATE AND TADALAFIL BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE-ARRAY DETECTION (UPLC-DAD). A simple ultra-performance liquid chromatographic method for the Simultaneous determination of sildenafil citrate and tadalafil was developed and validated. Sample preparation was dissolution in methanol, followed by centrifugation and dilution (1: 10) with methanol. Analysis was performed in in Acquity(R) UPLC system with Acquity(R) BEH C18 column (2.1 x 50 mm, with 1.7 mu m particles). The elution was isocratic with phosphate buffer pH 2.3 and acetonitrile (65:35, v/v) at a flow rate of 0.7 mL/min. The method presented adequate specificity, linearity, precision and accuracy and allowed the determination of the drugs in seized forensic samples.