DETERMINATION OF PHENOLIC COMPOUNDS BY ISOCRATIC HIGH PERFORMANCE LIQUID CHROMATOGRAFIC METHOD DURING STORAGE OF YERBA-MATE

摘要

DETERMINATION OF PHENOLIC COMPOUNDS BY ISOCRATIC HIGH PERFORMANCE LIQUID CHROMATOGRAFIC METHOD DURING STORAGE OF YERBA-MATE. Different phenolic compound, 5-caffeoylquinic acid (5-CQA), caffeic acid (AC) and rutin (Ru) contents of yerba-mate (Ilex paraguariensis) Brazilian samples of 06 different regions of Sao Mateus - Parana, during natural and accelerated industrial storage, were evaluated. For quantification, a reverse phase HPLC isocratic method was developed and validated using methanol: water (35: 65 v/v) acidified with 0.5% acetic acid as mobile phase and a photodiode array detector. The six sample global average contents were (34.90 and 36.10 mg g(-1)) for 5-CQA, (0.18 mg g(-1) and 0.23 mg g(-1)) for AC and (7.12 and 7.18 mg g(-1)) for Ru, respectively, for the natural and accelerated storage systems. The results showed that the 5-CQA and Ru content are kept constant during the storage while AC content increase only during accelerated storage.