OTIMIZACAO E VALIDACAO DE METODO MULTIRRESIDUO PARA DETERMINACAO DE SULFONAMIDAS EM CAMARAO CULTIVADO POR CROMATOGRAFIA LIQUIDA DE ALTA EFICIENCIA COM DETECCAO POR UV

摘要

This work reports the optimization and method validation for sulfonamides (sulfamethazine, sulfaquinoxaline, sulfadimethoxine and sulfathiazole) in shrimp muscle using HPLC-UV. The sulfonamides were extracted with acetonitrile and acetic acid, and the extract cleaned up with a Strata SCX SPE cartridge prior to analysis. The method presented linearity in the range of 20-120 μg kg~(-1), good linear correlation (r > 0.99), and limits of quantification in the range of 4.7-20.2 μg kg~(-1). The recovery for shrimp muscles spiked with 50-150 μg kg~(-1) ranged from 63.2-108.0%. Precision and accuracy analysis showed acceptable relative standard deviation. Commercial shrimps were analyzed and sulfonamides don't were found above of the method limit of quantification.