Determination of nimesulide by flow injection analysis with multiple-pulse amperometric detection

摘要

A simple and fast method for the determination of nimesulide (NI) using flow injection analysis with multiple-pulse amperometric (FIA-MPA) detection at a boron-doped diamond (BDD) electrode was developed. The method was based mainly on the application of a four-potential waveform, E_(1(det)) = -0.8 V / 30 ms, E_(2(det)) = 0.6 V / 30 ms, E_(3(det)) = -0.4 V / 30 ms and E_(4(cleaning)) = -0.45 V / 300 ms versus Ag/AgCl (3.0 mol L~(-1) KCl). NI was detected at three different electrode potentials, at which the nitro group undergoes different redox reactions. The proposed method was selective and sensitive (detection limit of 81.0 nmol L~(-1)), and successfully applied for the determination of NI in pharmaceutical formulations, yielding similar results to those obtained by the reference method.