Investigation of DNA cleavage activities of new oxime-type ligand complexes and molecular modeling of complex—DNA interactions

摘要

Nucleolytic activities of some new oxime-type ligand complexes were investigated by neutralagarose gel electrophoresis. Analysis of the cleavage products in agarose gel indicated that allcomplexes used converted supercoiled pUC18 plasmid DNA to its nicked or linear form. It was foundthat nucleolytic activities of the complexes depend on the complex concentration, reaction time andthe presence of a cooxidant (magnesium monoperoxyphthalate, MMPP) in the reaction mixture.However, the complexes cleaved pUC18 plasmid DNA at all investigated pH values. Nucleolyticactivities of complexes were investigated for different complex concentrations (0.1-100 1.1.mol L-1),pH values (6.0-10.0) and reaction times (0-60 min). Molecular modeling studies performed by theHyperchem Software together with DNA-binding studies showed that planar sites of the complexesintercalated into double stranded DNA. It can be concluded that all oxime-type ligand complexesused can be evaluated as nuclease mimics.