Non-invasive scanning ion-selective electrode technique and its applications to the research of higher plants

摘要

The process of various ions and molecules getting into and out of cells is critical for plant survival. The non-invasive scanning ion-selective electrode technique (SIET) is a non-invasive method to obtain the information of ions/molecules across membranes in plant. This technique can measure the absolute concentration of ions and molecules, and also their fluxes and directions of movement. The samples to be analyzed can be a single cell, a piece of tissue, a whole organ and even an intact seedling.This article reviews the recent progress made in plant physiology by using this technique and discusses its potentials in future studies on plant physiology.