NUCLEIC ACID AFFINITY CHROMATOGRAPHY - PREPARATION AND CHARACTERIZATION OF DOUBLE-STRANDED RNA AGAROSE

摘要

An improved method for the preparation of a double-stranded RNA-coupled resin is described. Periodate-oxidized double-stranded RNA, in the form of either purified reovirus double-stranded RNA or poly(rl) poly(rC), was covalently attached to agarose-adipic acid hydrazide, The resulting affinity resin had increased specificity for known double-stranded RNA binding proteins, as compared to a similar commercially available resin. The application of this resin in the purification and characterization of double-stranded RNA binding proteins is described. (C) 1995 Academic Press, Inc. [References: 25]