Chaperone-like effects of a scFv antibody on the folding of human muscle creatine kinase

摘要

The folding of polypeptide chains both in vitro and in vivo does not always proceed in a productive manner and may lead to the formation of misfolded conformations or even aggregations lacking their inherent biological activities (Dobson, 2004; Saunders and Bottomley, 2009; Wittung-Stafshede, 2011). A number of human diseases, such as Alzheimer's, Huntington's, Parkinson's and Creutzfeldt-Jakob's diseases, are associated with the misfolding and aggregation of proteins inside or outside cells (Horwich, 2002; Heller, 2010). Thus, the study of the causes and mechanisms of protein misfolding and aggregation as well as the search for techniques favoring productive protein folding in vivo are important tasks for both biophysics and biomedical research.