Isolation and characterization on novel neurotoxins from south American rattlesnake Crotalus durissus terrificus; optimization of chromatographic procedure

摘要

Molecular exclusion HPLC chromatography on a Protein Pak SW 300 column and reversed phase HPLC on a μ-Bondapack C18 column were used to purify four novel neurotoxins from the crotoxin complex, and five crotoxin subunits (PLA_2 and crotapotin) as well as two crotamine isoforms. A single preparative reversed phase HPLC steps yielded a major basic myotoxin followed by the subunits of crotoxin (three PLA_2 isoforms and two crotapotin isoforms). This modified procedure may be useful for the rapid, large-scale purification of toxins from Crotalid venoms.