Structural and Kinetic Overview of the Carboxylesterase EST2 from Alicyclobacillus acidocaldarius: A Comparison with the Other Members ofthe HSL Family

摘要

Thermophilic and hyperthermophilic carboxylesterases (EC 3.1.1.1) are excellent model systems for studyingstructure function relationships as well as in vitro and in vivo evolution and possible biotechnological applications. In thispaper we review the main aspect of one of most studied microbial representative of the hormone sensitive lipase family(HSL), namely carboxylesterase 2 (EST2) from Alicyclobacillus acidocaldarius.