Crystal structure of an eIF4G-like protein from Danio rerio.

摘要

The gene LOC791917 Danio rerio (zebrafish) encodes a protein annotated in the UniProt knowledgebase as the "middle domain of eukaryotic initiation factor 4G domain containing protein b" (MIF4Gdb). Its molecular weight is 25.8 kDa, and it comprises 222 amino acid residues. BLAST searches revealed homologues of D. rerio MIF4Gdb in many eukaryotes including humans. The homologues and MIF4Gdb were identified as members of the Pfam family, MIF4G (PF02854), which is named after the middle domain of eukaryotic initiation factor 4G (eIF4G). eIF4G is a component of eukaryotic translationnal initiation complex, and contains binding sites for other initiation factors, suggesting its critical role in translational initiation.