Evaluation of the PDQ? centrifuge for preparing platelet rich, platelet poor and platelet free plasma samples for light transmission aggregometry and microparticle measurement

摘要

Light transmission aggregometry (LTA) is considered the gold standard for platelet function testing [1]. This test requires the preparation of platelet rich plasma (PRP) and platelet poor plasma (PPP) from each blood sample [2]. Preparation of PRP and PPP by standard protocols is time consuming and poorly standardized (in terms of choice of g values and time of centrifugation) normally taking around 30-45 minutes [3-5]. There is now also much interest in the measurement of microparticles within platelet free plasma preparations (PFP) [6]. Again the preparation of PFP is also poorly standardized but is important for eliminating the generation of cell derived microparticles from any residual contaminating platelets or other cells during sample storage [6].