Effects of polymer topology upon transfection efficiency: synthesis, characterization, and transfection of poly-2-(dimethylamlno)ethyl methacrylate and poly-2-(dimethylamino)ehtyl-co-polyethylene glycol methacrylate

摘要

The promise of gene therapy involves the treatment of inherited or acquired genetic disorders through the transfection of an encoding DNA sequence into a target cell, which allows for the synthesis of a therapeutic protein. Due to the negatively charged phosphate backbone of DNA, direct injection of naked DNA hardly passes through cellular membranes and has the increased vulnerability of degradation by the host cell immune response. Gene transfer agents, carrier molecules, transport genetic material (DNA or RNA) and allows for more efficient Iran section. Significant research has been devoted to the design of viral gene transfer agents, which have remarkable transfection efficiencies.