首页> 外文期刊>Peptides: An International Journal >Interaction of antimicrobial peptides with bacterial polysaccharides from lung pathogens.
【24h】

Interaction of antimicrobial peptides with bacterial polysaccharides from lung pathogens.

机译:抗菌肽与肺病原体细菌多糖的相互作用。

获取原文
获取原文并翻译 | 示例
       

摘要

The interaction of two cathelicidin antimicrobial peptides, LL-37 and SMAP-29, with three bacterial polysaccharides, respectively, produced by Pseudomonas aeruginosa, Burkholderia cepacia and Klebsiella pneumoniae, was investigated to identify possible mechanisms adopted by lung pathogens to escape the action of innate immunity effectors. In vitro assays indicated that the antibacterial activity of both peptides was inhibited to a variable extent by the three polysaccharides. Circular dichroism experiments showed that these induced an alpha-helical conformation in the two peptides, with the polysaccharides from K. pneumoniae and B. cepacia showing, respectively, the highest and the lowest effect. Fluorescence measurements also indicated the presence of peptide-polysaccharide interactions. A model is proposed in which the binding of peptides to the polysaccharide molecules induces, at low polysaccharide to peptide ratios, a higher order of aggregation, due to peptide-peptide interactions. Overall, theseresults suggest that binding of the peptides by the polysaccharides produced by lung pathogens can contribute to the impairment of peptide-based innate defenses of airway surface.
机译:研究了两种Cathelicidin抗菌肽LL-37和SMAP-29与分别由铜绿假单胞菌,洋葱伯克霍尔德菌和肺炎克雷伯菌产生的三种细菌多糖的相互作用,以确定肺病原体采取的逃避先天性作用的可能机制。免疫效应物。体外测定表明,这三种肽的抗菌活性在三种多糖的作用下均受到不同程度的抑制。圆二色性实验表明,它们在两种肽中诱导了α-螺旋构象,肺炎克雷伯氏菌和洋葱伯克霍尔德氏菌的多糖分别显示出最高和最低的作用。荧光测量还表明存在肽-多糖相互作用。提出了一种模型,其中由于肽-肽相互作用,肽与多糖分子的结合以低多糖与肽的比率诱导更高的聚集级。总体而言,这些结果表明,肺病原体产生的多糖与多肽的结合可导致基于肽的气道表面先天防御功能受损。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号