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Study of the effect of 26RF- and 43RF-amides on Testosterone and Prolactin secretion in the adult male rhesus monkey (Macaca mulatta)

机译:26RF和43RF酰胺对成年雄性恒河猴(猕猴)睾丸激素和催乳激素分泌的影响的研究

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RF-amides (RFa), a superfamily of evolutionary-conserved neuropeptides, are expressed in both invertebrates and vertebrates. While some endocrine functions have been attributed to these peptides in lower vertebrates and few mammalian models, not much is known about their actions in primates. Therefore, the present study was designed to examine the effects of peripheral administration of two recently cloned human RFa peptides, 26RFa and 43RFa, on testosterone and prolactin secretion in the adult male adult male rhesus monkey (Macaca mulatta). For control purposes, a scrambled sequence of 26RFa (Sc-26RFa) and normal saline (1 ml) were injected. Three different doses of 26RFa and 43RFa (19-nmol, 38-nmol and 76-nmol) and a single dose (38-nmol) of Sc-26RFa were tested. A set of four chair-restraint habituated monkeys was used. Comparison of post-treatment T levels with respective pre levels showed that none of the doses of both 26RFa and 43RFa changed T release. Similarly, Sc-26RFa and saline administration also did not affect T levels. In contrast, all doses of 26RFa and 43RFa significantly (P < 0.05) stimulated prolactin secretion. 43RFa dose dependently increased prolactin secretion while dose dependency was not observed for 26RFa. Saline and Sc-26RFa injection had no effect on prolactin concentrations. Thus, present study demonstrated that peripheral administration of 26RFa and 43RFa, in the doses tested, have no effect on T secretion, suggesting possible selective lack of their neuroendocrine role in controlling hypothalamic-pituitary-gonadal axis in the adult male primates. The prominent stimulation of prolactin suggests a neuroendocrine role of RFa peptides in regulation of prolactin release in primates.
机译:RF-酰胺(RFa)是进化保守神经肽的超家族,在无脊椎动物和脊椎动物中都有表达。尽管在低等脊椎动物和少数哺乳动物模型中这些肽具有某些内分泌功能,但对其在灵长类动物中的作用知之甚少。因此,本研究旨在检查外围施用两种最近克隆的人RFa肽26RFa和43RFa对成年成年雄性恒河猴(Macaca mulatta)睾丸激素和催乳激素分泌的影响。为了控制目的,注射了加扰序列的26RFa(Sc-26RFa)和生理盐水(1 ml)。测试了三种不同剂量的26RFa和43RFa(19-nmol,38-nmol和76-nmol)和单剂量(38-nmol)的Sc-26RFa。使用一组四只约束椅子的习惯性猴子。治疗后的T水平与相应的前水平的比较表明,26RFa和43RFa的剂量均未改变T释放。同样,Sc-26RFa和生理盐水的给药也不会影响T水平。相反,所有剂量的26RFa和43RFa均显着(P <0.05)刺激催乳素分泌。 43RFa剂量依赖性增加催乳素分泌,而26RFa则未观察到剂量依赖性。盐水和Sc-26RFa注射液对催乳素浓度没有影响。因此,本研究表明,在所测试的剂量下,外周给药26RFa和43RFa对T分泌没有影响,表明在成年雄性灵长类动物中可能缺乏选择性地缺乏其神经内分泌作用来控制下丘脑-垂体-性腺轴。催乳素的显着刺激提示RFa肽在灵长类动物催乳素释放的调节中具有神经内分泌作用。

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