Novel process for enzymatic hydrolysis of proteins in an aqueous two-phase system for the production of peptide mixture

摘要

A novel process for the production of peptide mixtures is proposed. Biologically active peptides were synthesized using a thermolysin-catalyzed hydrolysis of a corn protein (zein) in an aqueous two-phase system. The mixture of peptides which was selectively recovered from the dextran-rich bottom phase had a higher angiotensin-converting enzyme (ACE) inhibitory activity than native zein.